lac-Repressor headpiece constitutes a reversibly unfolding domain
نویسندگان
چکیده
منابع مشابه
Characterization of mutations in oligomerization domain of Lac repressor protein.
A series of mutant lac repressor proteins at positions 281 or 282 was isolated for detailed characterization. Although Cys281 modification by sulfhydryl reagents abrogates pH effects on inducer binding and diminishes operator binding (Daly, T. J., Olson, J. S., and Matthews, K. S. (1986) Biochemistry 25, 5468-5474), substitution at this site by alanine, serine, phenylalanine, isoleucine, or met...
متن کاملIsolation of the lac repressor.
The realization that the synthesis of proteins is often under the control of repressorsl' 2 has posed a central question in molecular biology: What is the nature of the controlling substances? The scheme of negative control proposed by Jacob and Mionod envisages that certain genes, regulatory genes, make products that can act through the cytoplasm to prevent-the functioning of other genes. Thes...
متن کاملModeling the Lac repressor-operator assembly: the influence of DNA looping on Lac repressor conformation.
Repression of transcription of the Escherichia coli Lac operon by the Lac repressor (LacR) is accompanied by the simultaneous binding of LacR to two operators and the formation of a DNA loop. A recently developed theory of sequence-dependent DNA elasticity enables one to relate the fine structure of the LacR-DNA complex to a wide range of heretofore-unconnected experimental observations. Here, ...
متن کاملA single mutation in the core domain of the lac repressor reduces leakiness
BACKGROUND The lac operon provides cells with the ability to switch from glucose to lactose metabolism precisely when necessary. This metabolic switch is mediated by the lac repressor (LacI), which in the absence of lactose binds to the operator DNA sequence to inhibit transcription. Allosteric rearrangements triggered by binding of the lactose isomer allolactose to the core domain of the repre...
متن کاملlac Operator nucleosomes. 2. lac Nucleosomes can change conformation to strengthen binding by lac repressor.
We have shown previously that lac repressor binds specifically and quantitatively to lac operator restriction fragments which have been complexed with histones to form artificial nucleosomes (203 base pair restriction fragment) or core particles (144 base pair restriction fragment. We describe here a quantitative method for determining the equilibrium binding affinities of repressor for these l...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1981
ISSN: 0014-5793
DOI: 10.1016/0014-5793(81)80175-5